To assess the expression status of the neddylation pathway in pancreatic cancer, TCGA RNA-Seq dataset was performed to analyze the expression levels of NEDD8-activating enzyme E1 (NAE1) and NEDD8-conjugating enzyme E2 (Ubc12), E3 ubiquitin ligase Skp1/Cullin/F-box (Rbx1), NEDD8 in tumor vs. normal comparison, and patient overall survival. This evidence concerns the gene UBE2M and familial pancreatic carcinoma.