The top 20 genes with the highest mutation rates were illustrated, covering 92.68% of HCC patients (Figure 2A), including genes such as TP53 (56%), LRP1B (24%), CTNNB1 (22%), ATM (15%), APC (12%), ARID1A (12%), AXIN1 (12%), NOTCH3 (12%), TSC2 (12%), and ARID2 (10%). This evidence concerns the gene TSC2 and hepatocellular carcinoma.