In their exploration, what we need to pay more attention to is that BRAF V600E mutant organoids, together with the CIN pathway-associated gene mutations APC deletion and TP53 mutation in coculture, could reduce ERK/MAPK pathway activity, which improved the organoid environment and restored the growth and proliferation function of cells in BRAF V600E mutant organoids to some extent. This evidence concerns the gene APC and cervical squamous intraepithelial neoplasia.