When the basal levels of CD133 were determined in colorectal carcinoma cell line HCT116 and Colo205, pancreatic carcinoma cell line PANC-1 and MIA PaCa-2, and breast carcinoma cell line MCF7, HCT116 showed the highest level of CD133 (Supplementary Figures 1A, B, 2B). This evidence concerns the gene PROM1 and exocrine pancreatic carcinoma.