CD86 and neoplasm: We first analyzed the different immune cells, including CD4+ T cells (CD45+, CD3+, and CD4+), CD8+ T cells (CD45+, CD3+, and CD8+), myeloid-derived suppressor cells (MDSCs) (CD45+, CD11b+, and Gr-1+), M1 type tumor-associated macrophages (M1 cells) (CD45+, CD11b+, F4/80+, and CD86+), and M2 type tumor-associated macrophages (M2 cells) (CD45+, CD11b+, F4/80+, and CD206+) in the tumor immune microenvironment of control and RT groups by flow cytometry.