On the other hand, radiotherapy increases the effect of ICI: although CTLA-4 and PD-1/PD-L monoclonal antibody can reverse the inhibition of T cells in the immunosuppressive tumor microenvironment to some extent, the activation of T cells still depends on antigen stimulation and participation of activated costimulatory molecule CD28 on the surface of mature APC. This evidence concerns the gene CD28 and neoplasm.