To determine whether KLF5 was the direct functional mediator of α-Catulin-driven stem cell-like properties in NSCLC, we synthesized short-hairpin RNAs (shRNAs) for KLF5 and transduced these shRNAs into CL1-0 and A549 cells overexpressing α-Catulin. The gene discussed is KLF5; the disease is non-small cell lung carcinoma.