In conclusion, our present investigation confirms that cotreatment of CP-25 and 5-Fu synergistically inhibited HCC cell proliferation, induced cell apoptosis, and reduced invasion and metastasis in vitro and in vivo. The synergistic effect of CP-25 and 5-Fu may be the accumulation of p53 and activation of p53-induced mitochondria apoptosis pathway (Fig.8). Here, TP53 is linked to hepatocellular carcinoma.