MLC1 and lung carcinoma: In summary, while DAPK is autophosphorylated at pSer308 during mitosis, which represents a regulatory mechanism that prevents cell death due to a low level of kinase activity, low levels of phosphorylation of the exogenous substrate MLC were still detectable following Noc-treatment (overnight) and for 60 min upon release from the pro-/metaphase arrest in cervical and lung cancer cells.