NEFH and Sertoli Cell-Only Syndrome: Immunohistochemical analyses showed that expression of NFH has significantly increased in Sertoli cell only syndrome and germ cell arrest biopsies in males (Mayerhofer et al., 1999).The results showed that during the recovery of rat testicular Leydig cells following ablation with ethane dimethanesulfonate,expression of NFH in Leydig cells increased,suggesting that NFH contributes to the proliferation of Leydig cells (O'Shaughnessy et al., 2008).