Subclone3 is derived from subclone2 through mutation gains in genes like POU2AF1, CCNE1, ROBO2, and MYH9. CCNE1 is an oncogene that has frequently been amplified in malignancies (Pils et al., 2014), and MYH9 is considered to promote growth and metastasis in CRC (Wang et al., 2019). Here, POU2AF1 is linked to colorectal carcinoma.