SMAD4 and intestinal cancer: With regard to the Non-P group, the top 10 mutated genes were TP53, APC, PIK3CA, SMAD4, NOTCH3, FAT1, ARID1A, KMT2D, RECQL4, and FBXW7. According to the different mutation classification categories, the missense mutation was the one that obtained the highest proportion in the bowel cancer samples in the presence or absence of germline variants.