To verify the potential in vivo effect of Siah2 on CCA progression, we subcutaneously transplanted a xenograft in immunodeficient NCG mice in LV-NC/LV-Siah2 group and did not find a significant difference (Figures 6A–C), indicating that Siah2 exerted a marginal in vivo effect on CCA cell growth. Here, SIAH2 is linked to cholangiocarcinoma.