To substantiate the idea that activation of glutamatergic neurons in the PVN plays a critical role in anxiety-like behaviors, rAVV-CaMKIIα-ChR2-GFP (ChR2-GFP) or rAVV-CaMKIIα-GFP (GFP) was injected into the PVN of two groups, respectively. This evidence concerns the gene CAMK2A and Anxiety.