Although none of the studies have yet tested the penetration of 5-BDBD into brain tissue, in the ischemic stroke model, intraperitoneal injection of 5-BDBD significantly reduced the activation of microglia and the surface expression of P2X4 in the cerebral infarction area, as well as the concentration in plasma and brain tissue to achieve neuroprotection [30]. This evidence concerns the gene P2RX4 and ischemic stroke.