In this study, we applied multiplex immunohistochemistry (mIHC) and flow cytometry to investigate the comprehensive profile of TCF1+Texprog and TCF1−Texterm in HNSCC, including their spatial distribution, prognostic value, cytokine secretion, and correlation with regulatory T cells (Tregs) in the TIME. The gene discussed is TCF7; the disease is head and neck squamous cell carcinoma.