HIF1A and neoplasm: As a consequence, CoCl2 treatment in cells could elicit an elevated expression of HIF1α, which mimics the intracellular bioenergetic alterations of tumor hypoxia.20 We first examined the expression of HIF1α by western blot at different time points in KYSE30 treated by 200 μM CoCl2 and found that the expression of HIF1α was elevated in a time-dependent pattern.