FaDu was also transfected with pcDNA-E2F1, shE2F1 (Supplemental Figure S1(a)), pcDNA-MNX1-AS1, or shMNX1-AS1 (Supplemental Figure S2(a)) to investigate the effects of E2F1 or MNX1-AS1 on hypopharyngeal carcinoma. This evidence concerns the gene MNX1 and hypopharyngeal carcinoma.