Incubation with acRoots decreased the expression of E2F1 and MNX1-AS1 (Figure 3(c,d)) in a dosage-dependent way, indicating that acRoots might regulate E2F1 and MNX1-AS1 to participate in the progression of hypopharyngeal carcinoma. This evidence concerns the gene E2F1 and hypopharyngeal carcinoma.