As shown in Figure 3b and 3C, the levels of p-AKT/AKT and p-mTOR/mTOR of si-LINC00665 group declined significantly compared with si-con group in MCF7 and MDA-MB-231 cells (P < 0.01), suggesting that LINC00665-siRNA inhibited the survival of breast cancer cells through inactivating the AKT/mTOR signaling pathway. This evidence concerns the gene AKT1 and breast cancer.