We found that Sirt6loxp/loxp;Cre− mice had significantly higher levels of secreted TNFα in the peritoneal cavity than Sirt6loxp/loxp;Cre + mice (Fig. 7H), confirming that SIRT6 deletion in macrophages ameliorates TNFα secretion in vivo during obesity. This evidence concerns the gene TNF and obesity disorder.