We confirmed that SP1 could bind to the BACE1 promoter region using the ChIP and dual-luciferase reporter assays, and observed that BACE1 was increased in AD rats, reduced by miR-212-3p overexpression, and augmented by SP1 overexpression, suggesting that miR-212-3p inhibited SP1 to suppress BACE1 transcription both in AD rats and cells. This evidence concerns the gene SP1 and Alzheimer disease.