Multivariate logistic regression analysis was used to calculate the probability of nonrecovery based on the [TIMP-2]*[IGFBP7] day 0 clinical risk prediction model: probability for nonrecovery = 1/(1 + e−z), z = − 2.451 + 0.397 * ([TIMP-2]*[IGFBP7] day 0) + 0.060 * nonrenal SOFA score + 1.043 * AKI diagnosed by the UO criteria + 0.978 * AKI stage 2–3. The gene discussed is TIMP2; the disease is acute kidney injury.