HMGB1 and neoplasm: To determine whether the in vivo growth of the tumor made up of WT cells was suppressed when the cells were mixed with HMGB1-knockout clones, a total of 1 × 106 cells consisting of a mixture of WT and G9 cells in two different ratios, 1:1 and 1:3, were s.c. transplanted to B6 mice (Fig. 4).