To determine whether the in vivo growth inhibition found in HMGB1-knockout clones was a general phenomenon caused by HMGB1 knockout, we established two HMGB1-knockout clones (3H6, 9D4) from CT26 colon tumor cells and compared their in vivo tumor growth in BALB/c mice with that of WT CT26 cells (Fig. 2b). This evidence concerns the gene HMGB1 and colonic neoplasm.