To assess whether a correctly functioning CFTR was essentialforthe intracellular transport correction in CF cells, RSV was testedin cells isolated from Cftr–/– mice to check the improvement of cholesterol mobilization in MNE.RSV (50 μM for 24 h) significantly lowered cholesterol accumulationin Cftr–/– MNE cells, demonstratingthat CFTR function is not critical for RSV efficacy. Here, CFTR is linked to cystic fibrosis.