PGRN treatment further increased the percentage of BrdU+/DCX+ cells in BrdU+ cells compared to the pMCAO + Vehicle group 14 days after cerebral ischemia (F(2, 12) = 122.69, p < 0.01; Figure 6C), indicating cerebral ischemia stimulates NSCs differentiating into neurons, PGRN promotes ischemia‐induced neuronal differentiation of NSCs. The gene discussed is DCX; the disease is brain ischemia.