For instance, fluorescein isothiocyanate (FITC) or AlexaFluor 488, measured in the same channel, were assigned to antibodies recognizing melanoma cell adhesion molecule (MCAM or CD146), sialic acid binding Ig-like lectin H (Siglec-H), and glucocorticoid-induced tumor necrosis factor receptor (GITR). The gene discussed is TNFRSF18; the disease is melanoma.