Our results showed that diets of β-carotin and green tea powder alleviated the symptoms of gouty arthritis in mice, reduced the levels of serum uric acid and three type of pro-inflammatory cytokines, i.e., interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), improved the gut microbiota profile, and reduced the metabolic levels of purines and pyrimidines. Here, TNF is linked to gout.