To determine if the effects of SN-401 observed in vivo in T2D mice are attributable to SWELL1-LRRC8 binding, as opposed to off-target effects, we next measured fasting blood glucose and glucose tolerance in HFD-T2D mice treated with either SWELL1-active SN-403 or SN-406 as compared to SWELL1-inactive Inactive 1 (all at 5 mg/kg/day × 4 days). This evidence concerns the gene LRRC8A and type 2 diabetes mellitus.