ChIP-qPCR analysis confirmed ERα recruitment to the RN7SL1 gene in MCF-7 cells (Fig. 8D) The promoter of RN7SL1 includes sequences upstream of the transcription start site, as well as A- and B-box motifs within the transcribed region; ERα reads were evident in both these areas in MCF-7 cells, whereas ERα is localized to the upstream region in Met 3 and, much more weakly, in the primary tumour biopsies; the significance of this difference is unclear. This evidence concerns the gene ESR1 and neoplasm.