Correspondingly, Tbx18-expressing ECDCs showed multipotent differentiation ability in vitro and immortalized epicardium CDCs (iECDCs) transplantation significantly promoted cardiogenesis and improved left ventricular function in the failing heart following MI in mice, which provide a method to isolate epicardial Tbx18+ cells and offer a candidate cell type for cell therapy in cardiovascular diseases. This evidence concerns the gene TBX18 and myocardial infarction.