To understand the role of FOXO1 binding to PID1 in GBM cell sensitivity to chemotherapy, we established sh-PID1-1 and sh-PID1-2 sequences, the efficiency of which was verified in GBM cells, with sh-PID1-1 selected for subsequent assays due to its superior silencing efficiency (Figure 6G). This evidence concerns the gene FOXO1 and glioblastoma.