Based on the results of network pharmacology analysis (Figure 4; Table 5), first, the top four genes (PRKCA/MAPK3/PRKCG/PLA2G4A) and PRKCE (an isoform of the large PKC family) were picked up for validation in the animal model of surgery-induced myocardial ischemia. This evidence concerns the gene PRRT2 and myocardial ischemia.