Furthermore, the relationship between SIGLEC15,TIGIT,LAG3,CTLA4, PDCD1LG2 immune checkpoints and Mapk14 was only verified by correlation analysis, and we need to further explore the mechanism of Mapk14 expression in colorectal cancer in vitro and vivo experiments, such as single cell RNA sequencing. The gene discussed is CTLA4; the disease is colorectal cancer.