TNC and metastatic malignant neoplasm in the lymph nodes: Finally, we performed a LASSO logistic regression analysis of the gene expression matrix of seven of the THCA cohort from the TCGA database by the presence or absence of lymph node metastasis to finalize the risk scores constructed for the five most relevant genes (EVA1A, SERPINA1, FN1, TNC, MXRA8).