To characterize hyperphosphorylated tau in 3xTg-AD mice, brain sections from each 3xTg-AD mouse at 4-, 12-, and 18-month timepoints were stained for phosphorylated tau species using antibodies against paired helical filament tau phosphorylated at serine 202 and threonine 205 (via antibody AT8). This evidence concerns the gene MAPT and Alzheimer disease.