In accordance with our cell line model, we assessed TIMP1 protein levels in serum and their corresponding EVs in patients with advanced CRC (i.e., CRC liver MET) and patients with CRC having local primary disease at the time of blood collection (CRC); healthy donors with no known disease conditions (HD) were used as a control group (n = 20/group). The gene discussed is TIMP1; the disease is colorectal carcinoma.