Of significance, our results demonstrated that, similar to natural infection and to other vaccines administered in the human population, immunization of C57BL/6 mice with two doses of MVA-CoV2-S vaccine candidate induces long-term, strong, and polyfunctional SARS-CoV-2 S-specific CD4+ and CD8+ T-cell memory immune responses, as well as long-term SARS-CoV-2-specific humoral immune responses (binding IgG and neutralizing antibodies) against parental Wuhan strain and several VoC. This evidence concerns the gene CD4 and infection.