In addition, immunofluorescence analysis showed the presence of Tregs (CD3+FOXP3+) and suppressive macrophages (GFP+CD206+) in S100a4−/− host gliomas (Fig. 6f), indicating that S100a4 is not required for lineage determination or cellular differentiation of these leukocytes. The gene discussed is MRC1; the disease is glioma.