To functionally determine whether glioma-associated S100a4−/− immune cells are more anti-tumorigenic, we performed in vitro functional assays using T cells and myeloid cells (glioma-associated myeloid cells: GAMS) isolated from B6(S100a4+/+), S100a4+/−, and S100a4−/− host gliomas (Fig. 7a). The gene discussed is S100A4; the disease is central nervous system cancer.