We further clarified the location of CCL21+ stromal cells in thyroid tissue of HT patients using multicolor staining with the CCL21 RNA probe and antibodies of protein markers for lymphocytes (CD45), BCs (CD20), TC (CD3E), blood vascular ECs (VWF), myofibroblasts or fibroblasts (COL1A1), and myofibroblasts or smooth muscle cells (ACTA2). The gene discussed is COL1A1; the disease is thyroid gland disorder.