We then performed single-cell segmentation and quantified the main cell types identified in the original study9 by applying expert-defined thresholds to the expression of markers representative of each population (CDX2, MUC1 or cytokeratin for tumour cells; CD34 or CD31 for endothelial cells; vimentin for stromal cells; CD11c for dendritic cells; CD38 for B cells; CD3 and CD4 for CD4+ T cells; CD3, CD4 and FOXP3 for Tregs; CD3 and CD8 for CD8+ T cells, CD68 for macrophages). The gene discussed is VIM; the disease is neoplasm.