As a third case study, we applied SIMPLI to the spatial analysis of mIF-derived images of a rectal cancer sample (CRC1, Supplementary Data 1) stained with anti CD8, PD1, Ki67, PDL1, CD68, GzB and 4’,6-diamidino-2-phenylindole (DAPI) antibodies (Supplementary Data 2). This evidence concerns the gene CD8A and rectal cancer.