Furthermore, in a syngeneic subcutaneous PC mouse model, the addition of anti-PD-1 antibodies (200 μg on day 0, 3, 6, 9, 12, 15, 18, and 21; clone RMP1–14) to the CD40 agonist (100 μg on day 3; clone FGK45) and gemcitabine (120 mg/kg on day 1) + nab-paclitaxel (120 mg/kg on day 1) doubled long-term survival compared with a CD40 agonist + gemcitabine + nab-paclitaxel (26% versus 12%). This evidence concerns the gene PDCD1 and pachyonychia congenita.