CPNE1 and hepatocellular carcinoma: To validate whether CPNE1 was overexpressed in LIHC cell lines, we tested the mRNA expression level of CPNE1 in L02 (a human normal liver cell line) and 5 human hepatoma cell lines (MHCC-97H, HepG2, Hep3B, Huh7 and HLF) by using RT-qPCR.