In osteosarcoma, downregulation of CPNE1 not only significantly impaired the proliferation and metastasis of Saos-2 cell and enhanced sensitivity to cisplatin and doxorubicin, but also changed the expression of genes related to ECM receptors-associated pathway, MAPK pathway, TGF-β pathway, apoptotic pathway and NOD-like receptor pathway [20]. The gene discussed is TGFB1; the disease is osteosarcoma.