To confirm this PLSCR1-mediated repression of HCMV MIE gene expression, we next evaluated the expression levels of the 72-kDa protein IE1 (UL123), which is one of the proteins encoded by the MIE genes, in HEL, 36T-3, and PLS1KO-A cells after HCMV infection by immunoblot analysis. This evidence concerns the gene PLSCR1 and cytomegalovirus infection.