Under the condition that > 97% depletion of CD8 T cells was achieved in the splenocytes (Fig. 5E, right panel), CD8 T cell depletion canceled the antitumor activity of K3-SPG-ISV on both the vaccinated and untreated sides, indicating that ISV systemically suppressed tumor growth in a CD8 T cell dependent manner (Fig. 5E, left and center panels). This evidence concerns the gene CD8A and neoplasm.