Collectively, these results suggested that syntenin-1 may regulate biogenesis and secretion of sEVs as well as miR-494-3p expression in sEVs; In addition, miR-494-3p could act as a major onco-miRNA in sEVs secreted from human lung cancer cells to induce cancer progression in the TME. The gene discussed is SDCBP; the disease is lung carcinoma.