In the present study, we demonstrated a key role for oncogenic Ras/syntenin-1 axis that can stimulate release of sEVs and loading of miR-494-3p into sEVs in human lung cancer cells, and define its role during cancer progression in sEVs-mediated intercellular communication between cancer cells and endothelial cells in TME. The gene discussed is SDCBP; the disease is lung cancer.