Analysis of Ca2+ levels using Fluo-4-AM confirmed lack of glaucoma-induced Ca2+ increase in pericytes from Cacna1c−/− retinas, which displayed basal Ca2+ levels similar to sham controls, whereas substantial intrapericyte Ca2+ levels were detected in Cacna1c+/+ retinas (Fig. 3 G and H). The gene discussed is CACNA1C; the disease is glaucoma.