In particular, RNA-seq analysis demonstrated high expression of CD44, integrin-α6 (ITGA6), Olig2, Sox2, nestin and S100A4 in all 14 GSC cultures analyzed (few exceptions were detected, as expected due to GBM heterogeneity, but they did not correlate with CLIC1 mRNA levels); CD133 was expressed in a subset of GBMs (6/14, including the low CLIC1-expressing GBM39), while CD15 was detected in only two GBMs. This evidence concerns the gene NES and glioblastoma.