We next examined PTPN14 expression in various HCC cell lines (HepG2, Huh7 and SK-Hep1) using qRT-PCR (Fig. 2A) and western blotting (Fig. 2B), and compared expression in these cells to that of a normal hepatic cell line (LO2). Here, PTPN14 is linked to hepatocellular carcinoma.