However, when H3122 lung cancer-derived EVs (positive for EpCAM) were added to a healthy donor plasma sample before the immunocapture experiment (at a final concentration of 6.25 × 107 EVs/μl), the same sample showed a high positive signal for anti-EpCAM beads, as well as an increased signal for tetraspanins (Fig. 4B, right). This evidence concerns the gene EPCAM and lung carcinoma.