To evaluate whether the unequal proportion of full-length proviruses across the CD4+ T cell subsets can be observed at early stages of infection, we sorted memory Tcm, Ttm, and Tem CD4+ T cells from 3 HIV-1– donors (Supplemental Figure 1) and infected them for 20 hours with the HIV-1 laboratory strain, NL4-3. This evidence concerns the gene CD4 and infection.