Surprisingly, the results of Western blot showed that Ito,f-related proteins (Kv4.2, Kv4.3, and KChIP2) in the heart of CKD rats were significantly lower than those in the sham group (Figure 1, G and H), and the results of IHC also showed that Kv4.2 and Kv4.3 were downregulated in the heart of CKD rats compared with sham group (Figure 1J). This evidence concerns the gene KCND3 and chronic kidney disease.